Journal: Genetics

Article Title: Time-Course Analysis of Early Meiotic Prophase Events Informs Mechanisms of Homolog Pairing and Synapsis in Caenorhabditis elegans

doi: 10.1534/genetics.117.204172

Figure Lengend Snippet: Timing of synapsis for autosomes. (A) Stacked bar graph indicating the percent of nuclei exhibiting the indicated patterns of SYP-1 localization associated with autosomes at each time point (in nuclei with S-phase labeled X chromosomes). For nuclei in the “short stretch” class [example shown in (B)], one or a few short stretches of SYP-1 were observed, together with a large SYP-1 aggregate not associated with chromosome axes; it is unclear whether such short stretches represent bona fide synapsis intermediates. The “

Article Snippet: Interfocus distances reported in were measured using Volocity software. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Distinct organization of DAPI-stained chromatin during early meiotic prophase substages allows alignment of data from different experiments. (A–D) Left: immunofluorescence images of individual nuclei stained for DNA (DAPI); meiotic chromosome axis protein HTP-3; and SC central-region protein SYP-1, illustrating the DAPI organization features used to identify and define the indicated substages.

Techniques: Labeling, Immunostaining, Generated

Journal: Genetics

Article Title: Time-Course Analysis of Early Meiotic Prophase Events Informs Mechanisms of Homolog Pairing and Synapsis in Caenorhabditis elegans

doi: 10.1534/genetics.117.204172

Figure Lengend Snippet: Timing of X-chromosome synapsis. (A) Stacked bar graph indicating the percent of nuclei exhibiting the indicated patterns of SYP-1 localization associated with the X-chromosome pair at each time point. In the dot class, the only SYP-1 associated with the X chromosomes is a small dot that has clearly not been extended at all [example shown in (E)]; nuclei with any elongated SYP-1 stretch up to half the length of a chromosome pair comprise the “

Article Snippet: Interfocus distances reported in were measured using Volocity software. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Distinct organization of DAPI-stained chromatin during early meiotic prophase substages allows alignment of data from different experiments. (A–D) Left: immunofluorescence images of individual nuclei stained for DNA (DAPI); meiotic chromosome axis protein HTP-3; and SC central-region protein SYP-1, illustrating the DAPI organization features used to identify and define the indicated substages.

Techniques: Two Tailed Test, Labeling

Journal: PLoS Pathogens

Article Title: A tRNA-derived fragment present in E . coli OMVs regulates host cell gene expression and proliferation

doi: 10.1371/journal.ppat.1010827

Figure Lengend Snippet: Confocal microscopy imaging of human HCT116 cells stained with cell tracker CMTPX (in red) and incubated with bacterial OMVs labelled with PKH67 (in green) for 2 h ( A ) or 18 h ( B ). Nuclei were stained with DAPI (in blue). The XZ and YZ projection of volocity shows that bacterial OMVs were internalized by HCT116 cells and localized mainly to the cytoplasm (see ).

Article Snippet: Volocity 4.2.1 software , Quorum Technologies , Technical resources/software/downloads/.

Techniques: Confocal Microscopy, Imaging, Staining, Incubation

Journal: PLoS Pathogens

Article Title: A tRNA-derived fragment present in E . coli OMVs regulates host cell gene expression and proliferation

doi: 10.1371/journal.ppat.1010827

Figure Lengend Snippet: STAR★ Methods—Key Resources.

Article Snippet: Volocity 4.2.1 software , Quorum Technologies , Technical resources/software/downloads/.

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, Reporter Assay, Bacteria, Western Blot, Plasmid Preparation, Software, Spectrophotometry, Real-time Polymerase Chain Reaction